m-mlv reverse transcriptase 5 × reaction buffer (Thermo Fisher)

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Thermo Fisher m-mlv reverse transcriptase 5 × reaction buffer
M Mlv Reverse Transcriptase 5 × Reaction Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5%C3%97+reaction+buffer+reverse+transcriptase/pm34656692-113-29-36
Average 90 stars, based on 1 article reviews

m-mlv reverse transcriptase 5 × reaction buffer - by Bioz Stars, 2026-09

90/100 stars

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Reverse Transcription:

Article Title: Production of nucleic acid
Article Snippet: .. Reverse transcription reaction mixture was prepared on ice: 20 μl—5× reaction buffer for Reverse Transcriptase (Fermentas); 10 μl—10 mM dNTP (Fermentas); 2.5 μl—40 u/μl RiboLock RNase inhibitor (Fermentas); 5 μl—20 μM pD_42 oligonucleotide (SEQ ID No: 8); 2.5 μl RevertAidTM Minus M-MuLV Reverse transcriptase (200 u/μl)(Fermentas); 55 μl nuclease-free water. ..

Article Title: Production of nucleic acid
Article Snippet: .. Modified nucleotide incorporation reaction mix was prepared on ice by mixing: 5 μl—5× reaction buffer for Reverse Transcriptase (Fermentas); 1.25 μl—40 u/μl RiboLock RNase inhibitor (Fermentas); 2.5 μl—1 mM biotin-dUTP (Fermentas); 40.95 μl nuclease-free water and 0.3 μl of purified (<2.7*109 molecules) TC. ..

Article Title: Production of nucleic acid
Article Snippet: .. Reverse transcription reaction mixture for selection was prepared on ice: 60 μl-5× reaction buffer for Reverse Transcriptase (Fermentas); 7.5 μl-40 u/□l RiboLock RNase inhibitor (Fermentas); 15 μl-20 μM pD—42 oligonucleotide (SEQ ID No: 8); 186 μl nuclease-free water and 1.8 μl of purified (<1.8*1010 molecules) TC (translation in Roche—RTS HY kit). ..

Article Title: Production of nucleic acid
Article Snippet: .. Reverse transcription reaction mixture was prepared on ice: 20 μl-5× reaction buffer for Reverse Transcriptase (Fermentas); 10 μl-10 mM dNTP (Fermentas); 2.5 μl-40 u/μl RiboLock RNase inhibitor (Fermentas); 5 μl-20 μM pD_42 oligonucleotide (SEQ ID No: 8); 2.5 μl RevertAidTM Minus M-MuLV Reverse transcriptase (200 u/μl)(Fermentas); 55 μl nuclease-free water. ..

Article Title: Production of nucleic acid
Article Snippet: .. Reverse transcription reaction mixture for selected mRNA/dsDNA complex was prepared on ice: 12 μl-5× reaction buffer for Reverse Transcriptase (Fermentas); 6 μl -10 mM dNTP (Fermentas); 1.5 μl-40 u/μl RiboLock RNase inhibitor (Fermentas); 0.3 μl-20 μM pD—42 oligonucleotide (SEQ ID No: 8); 1.5 μl RevertAidTM Minus M-MuLV Reverse transcriptase (200 u/μl)(Fermentas); 35.7 μl nuclease-free water. ..

Article Title: Production of nucleic acid
Article Snippet: .. Reverse transcription reaction mixture for selection was prepared on ice: 60 μl-5× reaction buffer for Reverse Transcriptase (Fermentas); 7.5 μl-40 u/μl RiboLock RNase inhibitor (Fermentas); 15 μl-20 μM pD—42 oligonucleotide (SEQ ID No: 8); 188 μl nuclease-free water-mixture divided into two tubes 2×135 μl and 0.9 μl of purified (<8*109 molecules) TC (translation in Roche—RTS HY kit) or 0.9 μl of purified (<8*109 molecules) TC (translation in Wako—WakoPURE) were added. ..

Article Title: Production of nucleic acid
Article Snippet: .. Modified nucleotide incorporation reaction mix was prepared on ice by mixing: 5 μl-5× reaction buffer for Reverse Transcriptase (Fermentas); 1.25 μl-40 u/μl RiboLock RNase inhibitor (Fermentas); 2.5 μl-1 mM biotin-dUTP (Fermentas); 40.95 μl nuclease-free water and 0.3 μl of purified (<2.7*109 molecules) TC. ..

Article Title: Production of nucleic acid
Article Snippet: .. Reverse transcription reaction mixture for selected mRNA/dsDNA complex was prepared on ice: 12 μl—5× reaction buffer for Reverse Transcriptase (Fermentas); 6 μl—10 mM dNTP (Fermentas); 1.5 μl—40 u/μl RiboLock RNase inhibitor (Fermentas); 0.3 μl—20 μM pD_42 oligonucleotide (SEQ ID No: 8); 1.5 RevertAidTM Minus M-MuLV Reverse transcriptase (200 u/μl)(Fermentas); 35.7 μl nuclease-free water. ..

Modification:

Purification:

Article Title: Production of nucleic acid
Article Snippet: .. Reverse transcription reaction mixture for selection was prepared on ice: 60 μl-5× reaction buffer for Reverse Transcriptase (Fermentas); 7.5 μl-40 u/μl RiboLock RNase inhibitor (Fermentas); 15 μl-20 μM pD—42 oligonucleotide (SEQ ID No: 8); 188 μl nuclease-free water-mixture divided into two tubes 2×135 μl and 0.9 μl of purified (<8*109 molecules) TC (translation in Roche—RTS HY kit) or 0.9 μl of purified (<8*109 molecules) TC (translation in Wako—WakoPURE) were added. ..

Selection:

Article Title: Production of nucleic acid
Article Snippet: .. Reverse transcription reaction mixture for selection was prepared on ice: 60 μl-5× reaction buffer for Reverse Transcriptase (Fermentas); 7.5 μl-40 u/μl RiboLock RNase inhibitor (Fermentas); 15 μl-20 μM pD—42 oligonucleotide (SEQ ID No: 8); 188 μl nuclease-free water-mixture divided into two tubes 2×135 μl and 0.9 μl of purified (<8*109 molecules) TC (translation in Roche—RTS HY kit) or 0.9 μl of purified (<8*109 molecules) TC (translation in Wako—WakoPURE) were added. ..

Review